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Methods in Molecular Medicine Ser.: Nonviral Vectors for Gene Therapy : Methods and Protocols by Mark A. Findeis (2010, Trade Paperback)

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Nonviral Vectors for Gene Therapy by Mark A. Findeis. Author Mark A. Findeis. Title Nonviral Vectors for Gene Therapy. Format Paperback.

About this product

Product Information

The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents. These topics are of very broad importance since gene therapy evolves from its mostly ac- emy-based experimental and clinical research to the ever increasing number of industry-driven programs directed toward commercial development. S- cessful introduction of nonviral gene therapy agents into the clinic should be expected to require rigorous manufacturing and analytical methods that readily meet the regulatory guidelines under which new drug candidates are reviewed for marketing approval. Exactly what those guidelines will prove to be c- tainly depends on the established guidelines for review of both biological and chemical therapeutics. Additionally, many new techniques are being devised and applied to gene therapy research; these techniques will be instrumental in developing and characterizing successful gene delivery agents. Nonviral Vectors for Gene Therapy: Methods and Protocols has two main sections. To start with, there is a series of chapters on specific protocols for the synthesis, characterization, and application of gene delivery agents. S- eral chapters address the topic of materials to bind with DNA to form the compact condensed phases that facilitate cellular delivery.

Product Identifiers

Publisher
Humana Press
ISBN-10
1617371459
ISBN-13
9781617371455
eBay Product ID (ePID)
109216833

Product Key Features

Author
Mark A. Findeis
Publication Name
Nonviral Vectors for Gene Therapy : Methods and Protocols
Format
Trade Paperback
Language
English
Publication Year
2010
Series
Methods in Molecular Medicine Ser.
Type
Textbook
Number of Pages
Xi, 399 Pages

Dimensions

Item Length
9in
Item Width
6in
Item Weight
21.3 Oz

Additional Product Features

Series Volume Number
65
Number of Volumes
1 Vol.
Lc Classification Number
Qh573-671
Reviews
"The book begins with a series of chapters covering specific protocols concerning gene delivery agents. These are followed by several review chapters that provide additional information on preparing and characterizing gene transfer agents. Particular focus is given to spectroscopic techniques. The final chapter provides a review of renal gene therapy. Chapters are well organized and often accompanied by helpful figures. Each chapter includes a list of references, an an index is provided. Contributors are from research facilities throughout the world. This book is highly recommended for collections serving researchers in the area of gene therapy." - E-Streams
Table of Content
Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier.- Cationic ?-Helical Peptides for Gene Delivery into Cells.- Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (L-lysine) Dendrimer with Plasmid DNA.- Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery.- Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers.- Use of Disulfide Cationic Lipids in Plasmid DNA Delivery.- Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein.- Coupling of Nuclear Localization Signals to Plasmid DNA.- Progress Toward a Synthetic Virus.- Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers.- Methods for Studying the Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery.- Characterization of DNA Condensates by Atomic Force Microscopy.- Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting.- Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA.- Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes.- Nonviral DNA Delivery from Polymeric Systems.- Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers.- Lyophilization of Nonviral Gene Delivery Systems.- Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes.- Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy.- Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry.- Light-Scattering Techniques for Characterization of Synthetic Gene TherapyVectors.- Renal Gene Therapy.
Copyright Date
2001
Topic
Clinical Medicine, Life Sciences / Cell Biology, Genetics
Dewey Decimal
616/.042
Intended Audience
Scholarly & Professional
Dewey Edition
21
Illustrated
Yes
Genre
Science, Medical

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Picture 1 of 1

Methods in Molecular Medicine Ser.: Nonviral Vectors for Gene Therapy : Methods and Protocols by Mark A. Findeis (2010, Trade Paperback)

About this product

Product Information

Product Identifiers

Product Key Features

Dimensions

Additional Product Features

Best Selling in Textbooks

Diagnostic and Statistical Manual of Mental Disorders DSM-5-TR by American Psychiatric Association (2022, Trade Paperback)

Self Heal by Design : The Role of Micro-organisms for Health by Barbara O'Neill (2018, Trade Paperback)

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Designing Data-Intensive Applications : The Big Ideas Behind Reliable, Scalable, and Maintainable Systems by Martin Kleppmann (2017, Trade Paperback)

Research Design : Qualitative, Quantitative, and Mixed Methods Approaches by J. David Creswell and John W. Creswell (2022, Trade Paperback)

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