Methods in Molecular Biology Ser.: Signal Transduction Protocols by Stephen J. Hill (1995, Book, Other)

Humana Press

0896032981

9780896032989

163277

Signal Transduction Protocols

Stephen J. Hill

Book, Other

English

Life Sciences / Cell Biology, Life Sciences / Biochemistry

1995

Science

316 Pages

9in

0.4in

6in

0 Oz

Vol. 41

Qp517.C45s555 1995

...this well-organized volume is clearly among the most viable protocol books published in this field and will turn out to be a helpful tool for many laboratories.-FEBS Letters ...well organzied...up to date...should be in each laboratory or at least in your department library for easy reference. -Biopharm

The Problems and Pitfalls of Radioligand Binding. Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor. Autoradiography of b1- and b2- Adrenoreceptors. In Situ Hybridization. Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes. Measurement of Adenylyl Cyclase Activity in Cell Membranes. The Measurement of Cyclic AMP Levels in Biological Preparations. Mass Measurements of Cyclic AMP Formation by Radioimmunoassay Enzyme Immunoassay and Scintillation Proximity Assay. Measurement of Cyclic GMP Formation. Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay. Purification and Activation of Cyclic GMP-Dependent Protein Kinase. The Analysis and Assay of Cyclic Neucleotide Phosphodiesterase Isoenzyme Activity. Separation of Labeled Inositiol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC). Mass Measurement of Key Phosphoinositide Cycle Intermediates. Measurement of Phospholipase C Activity in Brain Membranes. Measurement of Diacylglycerol: Origins of Receptor-Stimulated Diacylglycerol Accumulation. Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2. Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3. Single-Cell Calcium Imaging. Characterization of Calcium/Calmodulin-Stimulated Proteinase Kinase II. Protein Kinase C: Measurement of Translocation Activation and Role in Cellular Responses. The Measurement of Phospholipase D-Linked Signaling in Cells. Phospholipase A2 Activity. Techniques for the Measurement of Nitric Oxide. Index.

1995

Trade

95-002769

574.87/6

Methods in Molecular Biology Ser.

20

Yes