The focus of the study is the spin column design with different materials. Effects of the binding of DNA with silica, glass microfiber membrane, whatmann filter paper under different binding conditions are discussed. Silica-based media and glass fiber membrane have become the standard for DNA binding in the plasmid preparation process. Chaotropic salts facilitate binding of DNA to the media. This creates a convenient way to wash away contaminants prior to eluting the DNA with a neutral solution, such as TE Buffer or water. Filter paper pellets can easily be processed by vacuum filtration or centrifuge to yield desired quantities of DNA. The use of spin column is a rapid, economic and sensitive method for DNA isolation. The use of these extraction method is both time and cost saving. Average, standard deviation, and coefficient of variation have been calculated for three methods.
Produktkennzeichnungen
ISBN-10
384844707x
ISBN-13
9783848447077
eBay Product ID (ePID)
165581683
Produkt Hauptmerkmale
Produktart
Lehrbuch
Sprache
Englisch
Anzahl der Seiten
84 Seiten
Verlag
Lap Lambert Academic Publishing
Publikationsname
Spin Column Designing For Dna Extraction
Autor
Hina Munir
Format
Taschenbuch
Erscheinungsjahr
2012
Zusätzliche Produkteigenschaften
Hörbuch
No
Inhaltsbeschreibung
Paperback
Item Length
22cm
Item Height
5mm
Item Width
15cm
Item Weight
143g
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